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Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure ArchiMer
Bougaran, Gael; Rouxel, Catherine; Dubois, Nolwenn; Kaas, Raymond; Grouas, Sophie; Lukomska, Ewa; Le Coz, Jean-rene; Cadoret, Jean-paul.
Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...)
Tipo: Text Palavras-chave: Strain selection; Isochrysis; Neutral lipid; Ultra violet; Flux cytometry; Biofuel.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00085/19643/17418.pdf
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Huîtres et norovirus : une affinité particulière? ArchiMer
Le Guyader, Soizick; Maalouf, Haifa; Le Pendu, Jacques.
Shellfish can be a vector for human pathogens. Despite regulation based on enteric bacteria, shellfish are still implicated in viral outbreaks. Oysters are the most common shellfish associated with outbreaks, and noroviruses, which cause acute gastroenteritis, are the most frequently identified pathogen in these outbreaks. Analysis of shellfish-related outbreak data worldwide shows an unexpected high proportion of genogroup I strains. Recent studies performed in vitro, in vivo and in the environment indicate that oysters are not just a passive filter, but can selectively accumulate norovirus strains based on virus carbohydrate ligands shared with humans. These observations may help explain the GI/GII bias observed in shellfish-related outbreaks compared to...
Tipo: Text Palavras-chave: Huîtres; Norovirus; Ligand; Sélection de souches; Oysters; Norovirus; Ligand; Strain selection.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00152/26357/24514.pdf
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Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.] BJM
Fernandes Júnior,Paulo Ivan; Lima,Andréa Aparecida de; Passos,Samuel Ribeiro; Gava,Carlos Alberto Tuão; Oliveira,Paulo Jansen de; Rumjanek,Norma Gouvêa; Xavier,Gustavo Ribeiro.
This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Strain selection; Biological nitrogen fixation; Biotechnology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400045
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Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid Bragantia
Marinho,Rita de Cássia Nunes; Ferreira,Linnajara de Vasconcelos Martins; Silva,Aleksandro Ferreira da; Martins,Lindete Míria Vieira; Nóbrega,Rafaela Simão Abrahão; Fernandes-Júnior,Paulo Ivan.
ABSTRACT Cowpea is a very important crop to Brazilian Semi-Arid mainly small family-based farmers. Rhizobia inoculation is a practice, easy to use, and cheap technology that increases cowpea productivity. The aim of this study was to evaluate the efficiency of two new rhizobia isolates in greenhouse and field as well as classify them taxonomically. To bacterial identification the 16S rRNA gene of ESA 17 and ESA 18 isolates were sequenced. The greenhouse test was conducted with pots containing 3 L of soil and the bacterial isolates evaluated were ESA 17, ESA 18, BR 3267 or BR 3262 strains. A field experiment was implemented on a Vertisol in Juazeiro, Bahia State, to evaluate the cowpea growth and productivity. In this experiment, the peat-based inoculants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradyrhizobium; Inoculant; Biological nitrogen fixation; Strain selection; São Francisco River Valley.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200273
Registros recuperados: 4
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